Journal: Frontiers in Microbiology

Article Title: Attachment Patterns of Human and Avian Influenza Viruses to Trachea and Colon of 26 Bird Species – Support for the Community Concept

doi: 10.3389/fmicb.2019.00815

Figure Lengend Snippet: Representative histochemistry images of stained tracheae TMAs. Red color indicates virus/lectin staining. The cells were counterstained with hematoxylin (blue).

Article Snippet: Consecutive TMA sections were stained with either 4 μg/mL Maackia amurensis lectin II (MAA-II) (BioNordika AB, Stockholm, Sweden) with tropism for α2,3-linked SA or 2 μg/mL Sambucus nigra lectin (SNA) (BioNordika) with tropism for α2,6-linked SA from Vector Laboratories.

Techniques: Staining, Virus

Journal: Frontiers in Microbiology

Article Title: Attachment Patterns of Human and Avian Influenza Viruses to Trachea and Colon of 26 Bird Species – Support for the Community Concept

doi: 10.3389/fmicb.2019.00815

Figure Lengend Snippet: Representative histochemistry images of stained cola TMAs. Red color indicates virus/lectin staining. The cells were counterstained with hematoxylin (blue).

Article Snippet: Consecutive TMA sections were stained with either 4 μg/mL Maackia amurensis lectin II (MAA-II) (BioNordika AB, Stockholm, Sweden) with tropism for α2,3-linked SA or 2 μg/mL Sambucus nigra lectin (SNA) (BioNordika) with tropism for α2,6-linked SA from Vector Laboratories.

Techniques: Staining, Virus

Journal: Scientific Reports

Article Title: Characterization of avian influenza virus attachment patterns to human and pig tissues

doi: 10.1038/s41598-018-29578-1

Figure Lengend Snippet: Median scores of attachment of studied IAVs and lectins to human tissues.

Article Snippet: Tissue sections were stained with either 4 μg/mL MAA-II (BioNordika AB, Stockholm, Sweden) or 2 μg/mL SNA (BioNordika) from Vector Laboratories.

Techniques:

Journal: Scientific Reports

Article Title: Characterization of avian influenza virus attachment patterns to human and pig tissues

doi: 10.1038/s41598-018-29578-1

Figure Lengend Snippet: Median scores of attachment of studied IAVs and lectins to pig tissues.

Article Snippet: Tissue sections were stained with either 4 μg/mL MAA-II (BioNordika AB, Stockholm, Sweden) or 2 μg/mL SNA (BioNordika) from Vector Laboratories.

Techniques:

Journal: Journal of Neuromuscular Diseases

Article Title: Development of Assays to Measure GNE Gene Potency and Gene Replacement in Skeletal Muscle

doi: 10.3233/JND-221596

Figure Lengend Snippet: Lectin binding to GNE -deficient muscle and CHO cells. A. Lectin binding comparing human MB135 cells and GNE –/– MB135 cells at 5, 7, or 13 days after serum starvation. B. Percent reduction in Sia staining by MAA and SNA in GNE –/– MB135 cells compared to MB135 control. C. Lectin binding comparing Chinese hamster ovary (CHO) cells and Lec3 cells, a Gne -deficient CHO cell line. D. Percent reduction in Sia staining by MAA and SNA in Lec3 cells compared to normal CHO control. Errors are SD for n = 3–5/group (A–D). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 Maackia amurensis agglutinin, MAA; Sambucus nigra agglutinin, SNA, Peanut agglutinin, PNA; Erythrina cristagalli agglutinin, ECA

Article Snippet: HRP-conjugated Maackia amurensis agglutinin (MAA, a combined mixture of types I and II), HRP-conjugated Sambucus nigra (SNA-1) agglutinin, HRP-conjugated Erythrina cristagalli agglutinin (ECA), or HRP-conjugated Arachis hypogaea agglutinin (also peanut agglutinin, PNA) (all from EY Laboratories; San Mateo, CA) were diluted at 1:500 in 2% fish gelatin in PBS, and cells were incubated for one hour with the appropriate lectin.

Techniques: Binding Assay, Staining, Control